A bump-hole strategy for increased stringency of cell-specific metabolic labeling of rna
Date
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Journal ISSN
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Publisher
Corporación Universidad de la Costa
Abstract
Description
Profiling RNA expression in a cell-specific manner continues to be a grand challenge in biochemical research. Bioorthogonal nucleosides can be utilized to track RNA expression; however, these methods currently have limitations due to background and incorporation of analogs into undesired cells. Herein, we design and demonstrate that uracil phosphoribosyltransferase can be engineered to match 5-vinyluracil for cell-specific metabolic labeling of RNA with exceptional specificity and stringency.
Keywords
Peptides and proteins, Genetics, Labeling, Uracil, Imaging probes