Mce4F Mycobacterium tuberculosis protein peptides can inhibit invasion of human cell lines

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dc.creatorRodríguez, Deisy Carolina
dc.creatorOcampo, Marisol
dc.creatorVarela, Yahson
dc.creatorCurtidor, Hernando
dc.creatorPatarroyo, Manuel Elkin
dc.creatorPatarroyo, Manuel A.
dc.date2015
dc.date2020-05-25T23:57:53Z
dc.date.accessioned2023-11-21T17:13:03Z
dc.date.available2023-11-21T17:13:03Z
dc.descriptionThis work was aimed at studying the Mycobacterium tuberculosis H37Rv Rv3494c protein, taking into account that it belongs to the mammalian cell entry family (mce) which is thought to have important functions in the disease's pathogenesis. The protein was characterized in silico; its presence on mycobacterial surface was confirmed by immunoelectron microscopy. High-activity binding peptides (HABPs) were identified by binding assays with 125I; their ability to inhibit mycobacterial entry to two cell lines (U937 alveolar macrophages and A549 epithelial cells) was ascertained and their role in bacterial entry was confirmed by fluorescent microsphere internalization assay. This protein's predicted alpha-helix structure was confirmed by circular dichroism of its peptides. All HABPs inhibited mycobacterial entry to cells and that the 38379 peptide (201IDQAGPFLQAQIRAGGDIKSY220) had high binding ability and inhibited the mycobacterial entry to both cell lines assayed here. Rv3494c peptides 38370 (21LSVMAIFYLRLPATFGIGTY40), 38373 (81HMRLNSGTAIPSNVTATVRSY100) and 38379 (201IDQAGPFLQAQIRAGGDIKSY220) showed to be HABP and inhibited mycobacterial entry to A549 cells and peptide 38382 (261RPSFPALAASLANLGRVGVIY280) bind to U937 and inhibited the mycobacterial entry to this cell line; all of these sequences play an important role in cell line recognition and invasion, and may thus be considered in the search for prophylactic candidates against tuberculosis. © FEMS 2015.
dc.formatapplication/pdf
dc.identifierhttps://doi.org/10.1093/femspd/ftu020
dc.identifier2049632X
dc.identifierhttps://repository.urosario.edu.co/handle/10336/22763
dc.identifier.urihttp://repository-salesiana.heoq.net/handle/123456789/290823
dc.languageeng
dc.publisherOxford University Press
dc.relationhttps://www.scopus.com/inward/record.uri?eid=2-s2.0-84946762612&doi=10.1093%2ffemspd%2fftu020&partnerID=40&md5=334d2f8a88a7167484c835d7227d69f8
dc.rightsinfo:eu-repo/semantics/openAccess
dc.sourceinstname:Universidad del Rosario
dc.sourcereponame:Repositorio Institucional EdocUR
dc.subjectBacterial protein
dc.subjectCytoplasm protein
dc.subjectIodine 125
dc.subjectMce4f protein
dc.subjectUnclassified drug
dc.subjectAntiinfective agent
dc.subjectBacterial antigen
dc.subjectBiological product
dc.subjectMembrane protein
dc.subjectMycobacterium tuberculosis antigens
dc.subjectProtein binding
dc.subjectA-549 cell line
dc.subjectAlpha helix
dc.subjectArticle
dc.subjectBacterial virulence
dc.subjectBeta sheet
dc.subjectCell invasion
dc.subjectCircular dichroism
dc.subjectControlled study
dc.subjectHuman
dc.subjectHuman cell
dc.subjectImmunoelectron microscopy
dc.subjectInternalization
dc.subjectLung alveolus macrophage
dc.subjectMycobacterium tuberculosis
dc.subjectNonhuman
dc.subjectPriority journal
dc.subjectProtein binding
dc.subjectProtein expression
dc.subjectU-937 cell line
dc.subjectCell line
dc.subjectDrug effects
dc.subjectEndocytosis
dc.subjectEpithelium cell
dc.subjectMacrophage
dc.subjectMetabolism
dc.subjectMicrobiology
dc.subjectMycobacterium tuberculosis
dc.subjectPhysiology
dc.subjectProtein conformation
dc.subjectAnti-bacterial agents
dc.subjectBiological products
dc.subjectCell line
dc.subjectCircular dichroism
dc.subjectEndocytosis
dc.subjectEpithelial cells
dc.subjectHumans
dc.subjectMacrophages
dc.subjectMembrane proteins
dc.subjectMycobacterium tuberculosis
dc.subjectProtein binding
dc.subjectProtein conformation
dc.subjectHabp
dc.subjectInhibition and invasion assay
dc.subjectMycobacterium tuberculosis h37rv
dc.subjectRv3494c protein
dc.subjectTuberculosis
dc.subjectbacterial
dc.subjectimmunoelectron
dc.subjectAntigens
dc.subjectMicroscopy
dc.titleMce4F Mycobacterium tuberculosis protein peptides can inhibit invasion of human cell lines
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion
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